Research

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.

Highlighted

Open collaborative writing with Manubot

Daniel S. Himmelstein, Vincent Rubinetti, David R. Slochower, Dongbo Hu, Venkat S. Malladi, Casey S. Greene, Anthony Gitter

PLOS Computational Biology  ·  24 Jun 2019  ·  doi:10.1371/journal.pcbi.1007128

All

2025

Balancing heterochromatin: ASB7-mediated proteasomal control of H3K9me3 homeostasis

Xinyu Zhou, Jonathan M. G. Higgins, Fangwei Wang

Science China Life Sciences  ·  14 Nov 2025  ·  doi:10.1007/s11427-025-3136-4

Chronic stress in cancer development and progression

Xin Niu, Ting Wu, Linghui Zeng, Fangwei Wang, Weiguo Lv, Long Zhang, Fangfang Zhou

Science Bulletin  ·  01 Nov 2025  ·  doi:10.1016/j.scib.2025.09.034

DNA binding and mitotic phosphorylation: native mechanisms preventing polyglutamine protein aggregation

Haiyan Yan, Xinyu Zhou, Junyue Tao, Fangwei Wang

Science China Life Sciences  ·  23 Sep 2025  ·  doi:10.1007/s11427-025-3058-6

FOXM1 condensate: a therapeutic target for breast tumor growth and metastasis

Haiyan Yan, Fangwei Wang, Weiguo Lu

Science Bulletin  ·  01 Sep 2025  ·  doi:10.1016/j.scib.2025.04.043

The cAMP-PKA signaling initiates mitosis by phosphorylating Bora

Min Zhu, Shinan Zhou, Yingqi Zhang, Qinfu Chen, Xueying Yuan, Long Zhang, Haiyan Yan, Fangwei Wang

Nature Communications  ·  24 Aug 2025  ·  doi:10.1038/s41467-025-63352-y

Scalable data harmonization for single-cell image-based profiling with CytoTable

Dave Bunten, Jenna Tomkinson, Erik Serrano, Michael J. Lippincott, Kenneth I. Brewer, Vince Rubinetti, Faisal Alquaddoomi, Gregory P. Way

openRxiv  ·  25 Jun 2025  ·  doi:10.1101/2025.06.19.660613

A chromatin-remodeling-independent role for ATRX in protecting centromeric cohesion

Lei Zhao, Xueying Yuan, Qinfu Chen, Haiyan Yan, Fangwei Wang

The EMBO Journal  ·  28 May 2025  ·  doi:10.1038/s44318-025-00465-6

Targeting a key disulfide linkage to regulate RIG-I condensation and cytosolic RNA-sensing

Bin Wang, Yongqiang Wang, Ting Pan, Lili Zhou, Yu Ran, …, Ting Liu, Huasong Lu, Bing Yang, Fangfang Zhou, Long Zhang

Nature Cell Biology  ·  14 Apr 2025  ·  doi:10.1038/s41556-025-01646-5

Decoding Mitotic Chromosome Assembly: Three Rules Governing Condensin–Cohesin Engagement

Haiyan Yan, Xinyu Zhou, Fangwei Wang

Research  ·  01 Jan 2025  ·  doi:10.34133/research.0812

Actin Takes the Steering Wheel: A New Mitotic Safeguard in the Early Embryo

Yingqi Zhang, Shukai Zhu, Fangwei Wang

Research  ·  01 Jan 2025  ·  doi:10.34133/research.1050

2024

Molecular mechanism and functional significance of Wapl interaction with the Cohesin complex

Xueying Yuan, Lu Yan, Qinfu Chen, Shukai Zhu, Xinyu Zhou, …, Jun Huang, Weiguo Lu, Long Zhang, Haiyan Yan, Fangwei Wang

Proceedings of the National Academy of Sciences  ·  07 Aug 2024  ·  doi:10.1073/pnas.2405177121

A non-canonical role of the inner kinetochore in regulating sister-chromatid cohesion at centromeres

Lu Yan, Xueying Yuan, Mingjie Liu, Qinfu Chen, Miao Zhang, …, Jun Huang, Weiguo Lu, Xiaojing He, Haiyan Yan, Fangwei Wang

The EMBO Journal  ·  07 May 2024  ·  doi:10.1038/s44318-024-00104-6

2023

The Monarch Initiative in 2024: an analytic platform integrating phenotypes, genes and diseases across species

Tim E Putman, Kevin Schaper, Nicolas Matentzoglu, Vincent P Rubinetti, Faisal S Alquaddoomi, …, Damian Smedley, Peter N Robinson, Christopher J Mungall, Melissa A Haendel, Monica C Munoz-Torres

Nucleic Acids Research  ·  24 Nov 2023  ·  doi:10.1093/nar/gkad1082

Integration of 168,000 samples reveals global patterns of the human gut microbiome

Richard J. Abdill, Samantha P. Graham, Vincent Rubinetti, Frank W. Albert, Casey S. Greene, Sean Davis, Ran Blekhman

openRxiv  ·  11 Oct 2023  ·  doi:10.1101/2023.10.11.560955

The RPA–RNF20–SNF2H cascade promotes proper chromosome segregation and homologous recombination repair

Jimin Li, Jingyu Zhao, Xiaoli Gan, Yanyan Wang, Donghao Jiang, …, Fangwei Wang, Jingyan Xu, Huadong Pei, Jun Huang, Xuefeng Chen

Proceedings of the National Academy of Sciences  ·  08 May 2023  ·  doi:10.1073/pnas.2303479120

MyGeneset.info: an interactive and programmatic platform for community-curated and user-created collections of genes

Ricardo Avila, Vincent Rubinetti, Xinghua Zhou, Dongbo Hu, Zhongchao Qian, Marco Alvarado Cano, Everaldo Rodolpho, Ginger Tsueng, Casey Greene, Chunlei Wu

Nucleic Acids Research  ·  18 Apr 2023  ·  doi:10.1093/nar/gkad289

The cGAS-STING pathway: a therapeutic target in chromosomally unstable cancers

Haiyan Yan, Weiguo Lu, Fangwei Wang

Signal Transduction and Targeted Therapy  ·  30 Jan 2023  ·  doi:10.1038/s41392-023-01328-4

Hetnet connectivity search provides rapid insights into how two biomedical entities are related

Daniel S. Himmelstein, Michael Zietz, Vincent Rubinetti, Kyle Kloster, Benjamin J. Heil, …, David N. Nicholson, Yun Hao, Blair D. Sullivan, Michael W. Nagle, Casey S. Greene

openRxiv  ·  07 Jan 2023  ·  doi:10.1101/2023.01.05.522941

2022

Hetnet connectivity search provides rapid insights into how biomedical entities are related

Daniel S Himmelstein, Michael Zietz, Vincent Rubinetti, Kyle Kloster, Benjamin J Heil, …, David N Nicholson, Yun Hao, Blair D Sullivan, Michael W Nagle, Casey S Greene

GigaScience  ·  28 Dec 2022  ·  doi:10.1093/gigascience/giad047

Role of pyroptosis in inflammation and cancer

Xiang Wei, Feng Xie, Xiaoxue Zhou, Yuchen Wu, Haiyan Yan, Ting Liu, Jun Huang, Fangwei Wang, Fangfang Zhou, Long Zhang

Cellular & Molecular Immunology  ·  15 Aug 2022  ·  doi:10.1038/s41423-022-00905-x

Dissecting the roles of Haspin and VRK1 in histone H3 phosphorylation during mitosis

Tyrell N. Cartwright, Rebecca J. Harris, Stephanie K. Meyer, Aye M. Mon, Nikolaus A. Watson, …, Agathe Marcelot, Fangwei Wang, Sophie Zinn-Justin, Paula Traktman, Jonathan M. G. Higgins

Scientific Reports  ·  01 Jul 2022  ·  doi:10.1038/s41598-022-15339-8

Microbiota in Tumors: From Understanding to Application

Yifan Xie, Feng Xie, Xiaoxue Zhou, Lei Zhang, Bing Yang, …, Fangwei Wang, Haiyan Yan, Linghui Zeng, Long Zhang, Fangfang Zhou

Advanced Science  ·  23 May 2022  ·  doi:10.1002/advs.202200470

MolEvolvR: A web-app for characterizing proteins using molecular evolution and phylogeny

Faisal S Alquaddoomi, Joseph T Burke, Lo Sosinski, David A Mayer, Evan P Brenner, …, Vincent P Rubinetti, Shaddai Amolitos, Kellen M Reason, John B Johnston, Janani Ravi

openRxiv  ·  22 Feb 2022  ·  doi:10.1101/2022.02.18.461833

SUMOylation in Viral Replication and Antiviral Defense

Yao Fan, Xiang Li, Lei Zhang, Zhi Zong, Fangwei Wang, …, Linghui Zeng, Chong Zhang, Haiyan Yan, Long Zhang, Fangfang Zhou

Advanced Science  ·  21 Jan 2022  ·  doi:10.1002/advs.202104126

2021

ISGylation in Innate Antiviral Immunity and Pathogen Defense Responses: A Review

Mengdi Zhang, Jingxian Li, Haiyan Yan, Jun Huang, Fangwei Wang, Ting Liu, Linghui Zeng, Fangfang Zhou

Frontiers in Cell and Developmental Biology  ·  25 Nov 2021  ·  doi:10.3389/fcell.2021.788410

Engineering Extracellular Vesicles Enriched with Palmitoylated ACE2 as COVID‐19 Therapy

Feng Xie, Peng Su, Ting Pan, Xiaoxue Zhou, Heyu Li, …, Jun Huang, Haiyan Yan, Linghui Zeng, Long Zhang, Fangfang Zhou

Advanced Materials  ·  19 Oct 2021  ·  doi:10.1002/adma.202103471

Targeted Anti‐Tumor Immunotherapy Using Tumor Infiltrating Cells

Yifan Xie, Feng Xie, Lei Zhang, Xiaoxue Zhou, Jun Huang, Fangwei Wang, Jin Jin, Long Zhang, Linghui Zeng, Fangfang Zhou

Advanced Science  ·  18 Oct 2021  ·  doi:10.1002/advs.202101672

Bub1 and CENP-U redundantly recruit Plk1 to stabilize kinetochore-microtubule attachments and ensure accurate chromosome segregation

Qinfu Chen, Miao Zhang, Xuan Pan, Xueying Yuan, Linli Zhou, …, Jun Huang, Weiguo Lu, Tatsuo Fukagawa, Fangwei Wang, Haiyan Yan

Cell Reports  ·  01 Sep 2021  ·  doi:10.1016/j.celrep.2021.109740

A subcellular map of the human kinome

Haitao Zhang, Xiaolei Cao, Mei Tang, Guoxuan Zhong, Yuan Si, …, Zongping Xia, Tingbo Liang, Huasong Lu, Xin-Hua Feng, Bin Zhao

eLife  ·  14 May 2021  ·  doi:10.7554/eLife.64943

2020

Compressing gene expression data using multiple latent space dimensionalities learns complementary biological representations

Gregory P. Way, Michael Zietz, Vincent Rubinetti, Daniel S. Himmelstein, Casey S. Greene

Genome Biology  ·  11 May 2020  ·  doi:10.1186/s13059-020-02021-3

Mps1 dimerization and multisite interactions with Ndc80 complex enable responsive spindle assembly checkpoint signaling

Ping Gui, Divine M Sedzro, Xiao Yuan, Sikai Liu, Mohan Hei, …, Felix O Aikhionbare, Xinjiao Gao, Dongmei Wang, Xuebiao Yao, Zhen Dou

Journal of Molecular Cell Biology  ·  26 Mar 2020  ·  doi:10.1093/jmcb/mjaa006

2019

Centromere-localized Aurora B kinase is required for the fidelity of chromosome segregation

Cai Liang, Zhenlei Zhang, Qinfu Chen, Haiyan Yan, Miao Zhang, Linli Zhou, Junfen Xu, Weiguo Lu, Fangwei Wang

Journal of Cell Biology  ·  23 Dec 2019  ·  doi:10.1083/jcb.201907092

Histone H2A phosphorylation recruits topoisomerase II α to centromeres to safeguard genomic stability

Miao Zhang, Cai Liang, Qinfu Chen, Haiyan Yan, Junfen Xu, …, Xueying Yuan, Jingbo Liu, Shixian Lin, Weiguo Lu, Fangwei Wang

The EMBO Journal  ·  26 Nov 2019  ·  doi:10.15252/embj.2019101863

NudC-like protein 2 restrains centriole amplification by stabilizing HERC2

Min Li, Xiaoyang Xu, Jun Zhang, Min Liu, Wei Wang, …, Yunkun Lu, Fangwei Wang, Wei Liu, Tianhua Zhou, Yuehong Yang

Cell Death & Disease  ·  19 Aug 2019  ·  doi:10.1038/s41419-019-1843-3

Open collaborative writing with Manubot

Daniel S. Himmelstein, Vincent Rubinetti, David R. Slochower, Dongbo Hu, Venkat S. Malladi, Casey S. Greene, Anthony Gitter

PLOS Computational Biology  ·  24 Jun 2019  ·  doi:10.1371/journal.pcbi.1007128

Sequential compression of gene expression across dimensionalities and methods reveals no single best method or dimensionality

Gregory P. Way, Michael Zietz, Vincent Rubinetti, Daniel S. Himmelstein, Casey S. Greene

openRxiv  ·  11 Mar 2019  ·  doi:10.1101/573782

Aurora B kinase activity–dependent and –independent functions of the chromosomal passenger complex in regulating sister chromatid cohesion

Qi Yi, Qinfu Chen, Haiyan Yan, Miao Zhang, Cai Liang, Xingfeng Xiang, Xuan Pan, Fangwei Wang

Journal of Biological Chemistry  ·  01 Feb 2019  ·  doi:10.1074/jbc.RA118.005978

A positive feedback mechanism ensures proper assembly of the functional inner centromere during mitosis in human cells

Cai Liang, Zhenlei Zhang, Qinfu Chen, Haiyan Yan, Miao Zhang, Xingfeng Xiang, Qi Yi, Xuan Pan, Hankun Cheng, Fangwei Wang

Journal of Biological Chemistry  ·  01 Feb 2019  ·  doi:10.1074/jbc.RA118.006046

2018

NudCL2 is an Hsp90 cochaperone to regulate sister chromatid cohesion by stabilizing cohesin subunits

Yuehong Yang, Wei Wang, Min Li, Ya Gao, Wen Zhang, …, Xiaoyi Yan, Wei Liu, Fangwei Wang, Dingwei Chen, Tianhua Zhou

Cellular and Molecular Life Sciences  ·  27 Oct 2018  ·  doi:10.1007/s00018-018-2957-y

HP1 cooperates with CAF-1 to compact heterochromatic transgene repeats in mammalian cells

Haiyan Yan, Xingfeng Xiang, Qinfu Chen, Xuan Pan, Hankun Cheng, Fangwei Wang

Scientific Reports  ·  20 Sep 2018  ·  doi:10.1038/s41598-018-32381-7

WAC Promotes Polo-like Kinase 1 Activation for Timely Mitotic Entry

Feifei Qi, Qinfu Chen, Hongxia Chen, Haiyan Yan, Binbin Chen, …, Miao Zhang, Hankun Cheng, Zhenlei Zhang, Jun Huang, Fangwei Wang

Cell Reports  ·  01 Jul 2018  ·  doi:10.1016/j.celrep.2018.06.087

HP 1 links centromeric heterochromatin to centromere cohesion in mammals

Qi Yi, Qinfu Chen, Cai Liang, Haiyan Yan, Zhenlei Zhang, Xingfeng Xiang, Miao Zhang, Feifei Qi, Linli Zhou, Fangwei Wang

EMBO reports  ·  28 Feb 2018  ·  doi:10.15252/embr.201745484

2017

A kinase‐dependent role for Haspin in antagonizing Wapl and protecting mitotic centromere cohesion

Cai Liang, Qinfu Chen, Qi Yi, Miao Zhang, Haiyan Yan, …, Linli Zhou, Zhenlei Zhang, Feifei Qi, Sheng Ye, Fangwei Wang

EMBO reports  ·  14 Nov 2017  ·  doi:10.15252/embr.201744737

The N-Terminal Non-Kinase-Domain-Mediated Binding of Haspin to Pds5B Protects Centromeric Cohesion in Mitosis

Linli Zhou, Cai Liang, Qinfu Chen, Zhenlei Zhang, Bo Zhang, …, Youchen Guan, Xingfeng Xiang, Xiaoqing Zhang, Sheng Ye, Fangwei Wang

Current Biology  ·  01 Apr 2017  ·  doi:10.1016/j.cub.2017.02.019

Aurora-A promotes the establishment of spindle assembly checkpoint by priming the Haspin-Aurora-B feedback loop in late G2 phase

Fazhi Yu, Ya Jiang, Lucy Lu, Mimi Cao, Yulong Qiao, …, Terry Van Dyke, Fangwei Wang, Xuebiao Yao, Jing Guo, Zhenye Yang

Cell Discovery  ·  10 Jan 2017  ·  doi:10.1038/celldisc.2016.49

2014

Polo‐like kinase‐1 triggers histone phosphorylation by Haspin in mitosis

Linli Zhou, Xiaoying Tian, Cailei Zhu, Fangwei Wang, Jonathan MG Higgins

EMBO reports  ·  13 Jan 2014  ·  doi:10.1002/embr.201338080

2013

Histone modifications and mitosis: countermarks, landmarks, and bookmarks

Fangwei Wang, Jonathan M.G. Higgins

Trends in Cell Biology  ·  01 Apr 2013  ·  doi:10.1016/j.tcb.2012.11.005

2012

Haspin inhibitors reveal centromeric functions of Aurora B in chromosome segregation

Fangwei Wang, Natalia P. Ulyanova, John R. Daum, Debasis Patnaik, Anna V. Kateneva, Gary J. Gorbsky, Jonathan M.G. Higgins

Journal of Cell Biology  ·  15 Oct 2012  ·  doi:10.1083/jcb.201205106

Molecular basis for phosphospecific recognition of histone H3 tails by Survivin paralogues at inner centromeres

Ewa Niedzialkowska, Fangwei Wang, Przemyslaw J. Porebski, Wladek Minor, Jonathan M. G. Higgins, P. Todd Stukenberg

Molecular Biology of the Cell  ·  15 Apr 2012  ·  doi:10.1091/mbc.E11-11-0904

2011

A Positive Feedback Loop Involving Haspin and Aurora B Promotes CPC Accumulation at Centromeres in Mitosis

Fangwei Wang, Natalia P. Ulyanova, Maike S. van der Waal, Debasis Patnaik, Susanne M.A. Lens, Jonathan M.G. Higgins

Current Biology  ·  01 Jun 2011  ·  doi:10.1016/j.cub.2011.05.016

2010

A phospho/methyl switch at histone H3 regulates TFIID association with mitotic chromosomes

Radhika A Varier, Nikolay S Outchkourov, Petra de Graaf, Frederik M A van Schaik, Henk Jan L Ensing, Fangwei Wang, Jonathan M G Higgins, Geert J P L Kops, HTh Marc Timmers

The EMBO Journal  ·  15 Oct 2010  ·  doi:10.1038/emboj.2010.261

Histone H3 Thr-3 Phosphorylation by Haspin Positions Aurora B at Centromeres in Mitosis

Fangwei Wang, Jun Dai, John R. Daum, Ewa Niedzialkowska, Budhaditya Banerjee, P. Todd Stukenberg, Gary J. Gorbsky, Jonathan M. G. Higgins

Science  ·  08 Oct 2010  ·  doi:10.1126/science.1189435

2009

Structure and functional characterization of the atypical human kinase haspin

Jeyanthy Eswaran, Debasis Patnaik, Panagis Filippakopoulos, Fangwei Wang, Ross L. Stein, James W. Murray, Jonathan M. G. Higgins, Stefan Knapp

Proceedings of the National Academy of Sciences  ·  01 Dec 2009  ·  doi:10.1073/pnas.0901989106

2006

The Assembly and Maintenance of Heterochromatin Initiated by Transgene Repeats Are Independent of the RNA Interference Pathway in Mammalian Cells

Fangwei Wang, Naoki Koyama, Hiroko Nishida, Tokuko Haraguchi, Walter Reith, Toshiro Tsukamoto

Molecular and Cellular Biology  ·  01 Jun 2006  ·  doi:10.1128/MCB.02189-05